3D imaging of the vagus nerve fascicular anatomy with cryo-imaging and UV excitation

Chaitanya Kolluru; Ananya Subramaniam; Yehe Liu; Aniruddha Upadhye; Monty Khela; Lindsey Druschel; Farzad Fereidouni; Richard Levenson; Andrew Shoffstall; Michael Jenkins; David L. Wilson

doi:10.1117/12.2577037

5 March 2021 3D imaging of the vagus nerve fascicular anatomy with cryo-imaging and UV excitation

Chaitanya Kolluru, Ananya Subramaniam, Yehe Liu, Aniruddha Upadhye, Monty Khela, Lindsey Druschel, Farzad Fereidouni, Richard Levenson, Andrew Shoffstall, Michael Jenkins, David L. Wilson

Author Affiliations +

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164910 (2021) https://doi.org/10.1117/12.2577037
Event: SPIE BiOS, 2021, Online Only

Abstract

Vagus nerve stimulation (VNS) is a method to treat drug-resistant epilepsy and depression, but therapeutic outcomes are often not ideal. Newer electrode designs such as intra-fascicular electrodes offer potential improvements in reducing off-target effects but require a detailed understanding of the fascicular anatomy of the vagus nerve. We have adapted a section-and-image technique, cryo-imaging, with UV excitation to visualize fascicles along the length of the vagus nerve. In addition to offering optical sectioning at the surface via reduced penetration depth, UV illumination also produces sufficient contrast between fascicular structures and connective tissue. Here we demonstrate the utility of this approach in pilot experiments. We imaged fixed, cadaver vagus nerve samples, segmented fascicles, and demonstrated 3D tracking of fascicles. Such data can serve as input for computer models of vagus nerve stimulation.

Conference Presentation

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Chaitanya Kolluru, Ananya Subramaniam, Yehe Liu, Aniruddha Upadhye, Monty Khela, Lindsey Druschel, Farzad Fereidouni, Richard Levenson, Andrew Shoffstall, Michael Jenkins, and David L. Wilson "3D imaging of the vagus nerve fascicular anatomy with cryo-imaging and UV excitation", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164910 (5 March 2021); https://doi.org/10.1117/12.2577037

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