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5 March 2021 Single-cell analysis pipeline for quantification of intracellular drug distribution by CARS/2PF/FLIM
Jindou Shi, Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Darold Spillman Jr., Marina Marjanovic, Minh Doan, Steve R. Hood, Stephen A. Boppart
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Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164911 (2021) https://doi.org/10.1117/12.2577041
Event: SPIE BiOS, 2021, Online Only
Abstract
An efficient and automated image analysis pipeline is essential for extracting quantitative information from multimodal image datasets. In this study, a multimodal optical imaging platform was used to capture CARS, 2PF, and FLIM images from control and drug-treated cells. Images were collected using both fluorescent label-based and label-free approaches. Here we present a single-cell analysis pipeline for the multimodal cellular image analysis. The results demonstrate the capability of our single-cell analysis pipeline for quantitatively measuring the intracellular drug distribution and its longitudinal uptake using a multimodal optical imaging platform, which can provide novel insights into the uptake pathways and target-sites.
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Jindou Shi, Prabuddha Mukherjee, Aneesh Alex, Edita Aksamitiene, Darold Spillman Jr., Marina Marjanovic, Minh Doan, Steve R. Hood, and Stephen A. Boppart "Single-cell analysis pipeline for quantification of intracellular drug distribution by CARS/2PF/FLIM", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164911 (5 March 2021); https://doi.org/10.1117/12.2577041
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KEYWORDS
Fluorescence lifetime imaging
Image analysis
Optical imaging
CARS tomography
Luminescence
Microscopy
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