Real-time optical flow sensing with SD-OCT: towards a modular motion-correction platform for microscopy

Stephen Tucker; Michael Wallace; Jianbo Tang; Anderson Chen; David Boas

doi:10.1117/12.2578380

5 March 2021 Real-time optical flow sensing with SD-OCT: towards a modular motion-correction platform for microscopy

Stephen Tucker, Michael Wallace, Jianbo Tang, Anderson Chen, David Boas

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164917 (2021) https://doi.org/10.1117/12.2578380
Event: SPIE BiOS, 2021, Online Only

Conference Poster

Abstract

Scanning microscopy’s resolution of highly localized dynamics in awake animals is limited by bulk motion. We present a modular optical flow sensor based on spectral domain OCT which measures the specimen’s 3D displacement in real-time. A rose-function scan pattern rapidly acquires B-lines used to estimate displacements via an adaptive cross-correlation approach. Axial displacements are estimated from phase fluctuations apparent in the cross-correlation of the complex SD-OCT signal. The technique’s 3-dimensional readout rate and sensitivity to physiologically-relevant motion frequencies and magnitudes is evaluated.

Conference Presentation

Citation Download Citation

Stephen Tucker, Michael Wallace, Jianbo Tang, Anderson Chen, and David Boas "Real-time optical flow sensing with SD-OCT: towards a modular motion-correction platform for microscopy", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164917 (5 March 2021); https://doi.org/10.1117/12.2578380

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