A nano-illumination microscope with 7 mm2 extended field-of-view and resolution below 1µm

S. Moreno; J. Canals; V. Moro; N. Franch; A. Vilà; A. Romano; J. D. Prades; D. D. Bezshlyakh; A. Waag; S. Krieger; S. Geleff; A. Diéguez

doi:10.1117/12.2578225

5 March 2021 A nano-illumination microscope with 7 mm² extended field-of-view and resolution below 1μm

S. Moreno, J. Canals, V. Moro, N. Franch, A. Vilà, A. Romano, J. D. Prades, D. D. Bezshlyakh, A. Waag, S. Krieger, S. Geleff, A. Diéguez

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Proceedings Volume 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics; 116510P (2021) https://doi.org/10.1117/12.2578225
Event: SPIE BiOS, 2021, Online Only

Abstract

In this work we present a new microscope based on Nano-illumination microscopy (NIM), i.e., an innovative technique based on a 2D array of nano-Light-Emitting Diodes (LEDs) used to illuminate a sample. The key point of this method is that the pitch of the LED array fixes the spatial resolution. So, potentially, with LED pitches lower than the diffraction limit, super resolution could be achieved. While nanometer sized LEDs are not available yet, we present a prototype based on optical downscaling of a single 5µm lateral size LED. Extended Field-of-View (FOV) is obtained by mechanical movement with nanopositioners. Aspects of NIM microscopy such as its size, its flexibility in the sensing hardware or its potential for fluorescence, make it a perfect candidate to enhance emerging sensing applications in different fields, but especially life science (medical imaging, genomics, ...). We demonstrate the possibilities of the NIM technique with patterns as well as with biological samples.

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S. Moreno, J. Canals, V. Moro, N. Franch, A. Vilà, A. Romano, J. D. Prades, D. D. Bezshlyakh, A. Waag, S. Krieger, S. Geleff, and A. Diéguez "A nano-illumination microscope with 7 mm² extended field-of-view and resolution below 1μm", Proc. SPIE 11651, Optical Diagnostics and Sensing XXI: Toward Point-of-Care Diagnostics, 116510P (5 March 2021); https://doi.org/10.1117/12.2578225

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