Compact, easy-to-use adaptive optics module for light-sheet structural and functional imaging of neuronal structures

Fabrice Harms; Antoine Hubert; Pauline Treimany; Cynthia Veilly; Guillaume Dovillaire; Xavier Levecq; Vincent Loriette; Alexandra Fragola

doi:10.1117/12.2583287

5 March 2021 Compact, easy-to-use adaptive optics module for light-sheet structural and functional imaging of neuronal structures

Fabrice Harms, Antoine Hubert, Pauline Treimany, Cynthia Veilly, Guillaume Dovillaire, Xavier Levecq, Vincent Loriette, Alexandra Fragola

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Proceedings Volume 11652, Adaptive Optics and Wavefront Control for Biological Systems VII; 116520K (2021) https://doi.org/10.1117/12.2583287
Event: SPIE BiOS, 2021, Online Only

Abstract

We report a compact adaptive optics (AO) module with optimized optical design and photon budget, compatible with multiple wavelengths, and adaptable to most of existing Light-Sheet setups, enabling a 2 to 3-fold signal improvement on neuronal structures of the live, non-clarified drosophila brain (neurons, projections), at depths ranging from 50 to 100µm. We report similar signal improvement brought by AO on functional signals from neurons of the drosophila circadian clock network. The proposed setup paves the way to fully automatized AO Light-Sheet systems usable in routine by biologists.

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Fabrice Harms, Antoine Hubert, Pauline Treimany, Cynthia Veilly, Guillaume Dovillaire, Xavier Levecq, Vincent Loriette, and Alexandra Fragola "Compact, easy-to-use adaptive optics module for light-sheet structural and functional imaging of neuronal structures", Proc. SPIE 11652, Adaptive Optics and Wavefront Control for Biological Systems VII, 116520K (5 March 2021); https://doi.org/10.1117/12.2583287

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