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5 March 2021 Laser scanning GLIM (LS-GLIM) for label-free imaging of turbid samples
Xi Chen, Mikhail Kandel, Shenghua He, Young Jae Lee, Kathryn Sullivan, Hyun Joon Kong, Mark Anastasio, Gabriel Popescu
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Proceedings Volume 11653, Quantitative Phase Imaging VII; 1165304 (2021) https://doi.org/10.1117/12.2584286
Event: SPIE BiOS, 2021, Online Only
Abstract
We propose a multimodal imaging system, LS-GLIM, consisting of the light scanning microscope (LSM 900, Zeiss) and the gradient light interference microscope (GLIM) module [1], with photomultiplier tube (PMT) detection. GLIM upgrades a differential interference microscope with a liquid crystal variable retarder (LCVR), which introduces further controlled phase shifts between the x and y polarized light. The quantitative phase information can be retrieved from the four frames corresponding to each phase shift in the GLIM. We anticipate a broad range of applications that LS-GLIM will enable through phase imaging with computational specificity (PICS), especially in thick, highly-scattering samples.
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Xi Chen, Mikhail Kandel, Shenghua He, Young Jae Lee, Kathryn Sullivan, Hyun Joon Kong, Mark Anastasio, and Gabriel Popescu "Laser scanning GLIM (LS-GLIM) for label-free imaging of turbid samples", Proc. SPIE 11653, Quantitative Phase Imaging VII, 1165304 (5 March 2021); https://doi.org/10.1117/12.2584286
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KEYWORDS
Laser scanners
Luminescence
Microscopes
Confocal microscopy
Laser sources
Light
Phase shifts
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