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5 March 2021 Laser scanning GLIM (LS-GLIM) for label-free imaging of turbid samples
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Abstract
We propose a multimodal imaging system, LS-GLIM, consisting of the light scanning microscope (LSM 900, Zeiss) and the gradient light interference microscope (GLIM) module [1], with photomultiplier tube (PMT) detection. GLIM upgrades a differential interference microscope with a liquid crystal variable retarder (LCVR), which introduces further controlled phase shifts between the x and y polarized light. The quantitative phase information can be retrieved from the four frames corresponding to each phase shift in the GLIM. We anticipate a broad range of applications that LS-GLIM will enable through phase imaging with computational specificity (PICS), especially in thick, highly-scattering samples.
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© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xi Chen, Mikhail Kandel, Shenghua He, Young Jae Lee, Kathryn Sullivan, Hyun Joon Kong, Mark Anastasio, and Gabriel Popescu "Laser scanning GLIM (LS-GLIM) for label-free imaging of turbid samples", Proc. SPIE 11653, Quantitative Phase Imaging VII, 1165304 (5 March 2021); https://doi.org/10.1117/12.2584286
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