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5 March 2021 Medical nanoparticle impact on macrophage temporal dry mass development quantified in vitro by digital holographic microscopy
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Abstract
Quantitative phase imaging (QPI) has been demonstrated to be a versatile tool for minimally invasive label-free imaging of biological specimens and time-resolved cellular analysis. In cytotoxicity testing, a commonly performed task in the preclinical development of new medical products, a flexible time-resolved label-free quantification of drug mediated effects is highly desirable. We thus explored the capabilities of digital holographic microscopy (DHM), an interferometric variant of QPI, to determine the temporal dry mass development of a cell population of RAW 264.7 mouse macrophages to quantify effects caused by medical nanoparticles in vitro in comparison to digitonin, which was used as cytotoxic positive control, and untreated cells in culture medium. Digital off-axis holograms from medical nanoparticles and digitonin incubated cells as well as from untreated control cells were acquired automated in multi-well imaging plates using a modular DHM system that was equipped with a motorized stage. From the retrieved time-lapse series of quantitative phase images, the temporal development of the dry mass in the observed field of view was calculated. As dry mass is correlated to cellular proliferation, toxicological effects of the medical nanoparticles on macrophages can be quantified at the nanogram scale. We found a similar temporal dry mass development of control macrophages in the cell culture medium and after incubation with non-toxic liposomes, while cytotoxic poly(alkyl cyanoacrylate) nanoparticles significantly reduced the dry mass increment in a dose-dependent manner. In summary, our results show for macrophages that the observation of the temporal dry mass development allows to discriminate reliably between non(low)-toxic and harmful nanomaterials.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kai Eder, Anne Marzi, Álvaro Barroso, Björn Kemper, and Jürgen Schnekenburger "Medical nanoparticle impact on macrophage temporal dry mass development quantified in vitro by digital holographic microscopy", Proc. SPIE 11653, Quantitative Phase Imaging VII, 116531Q (5 March 2021); https://doi.org/10.1117/12.2577655
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KEYWORDS
Digital holography

Nanoparticles

Holography

In vitro testing

Microscopy

Holographic interferometry

Interferometry

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