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5 March 2021 Label-free measurement of cell mechanical properties with digital holographic microscopy
Erik Bélanger, Sara Mattar, Sébastien Boutet, Gabrielle Jess, Isabelle St-Amour, Marie-Ève Crochetière, Jean-Xavier Giroux, Pierre Marquet
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Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550T (2021) https://doi.org/10.1117/12.2577348
Event: SPIE BiOS, 2021, Online Only
Abstract
Many studies suggest that external forces applied to cells generate signals that are as potent as those of biochemical stimuli. To understand how these forces are transmitted to the molecular structures of cells, and how they might be transduced into biochemical reactions, require measuring both cell mechanical properties (CMPs) and biological pathways in a physiological environment. For this purpose, we will present measurements, through rheological approaches, of CMPs in a label-free manner performed thanks to an automated imaging system devoted to live cells combining digital holographic microscopy, environmental control, and microfluidic assays.
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Erik Bélanger, Sara Mattar, Sébastien Boutet, Gabrielle Jess, Isabelle St-Amour, Marie-Ève Crochetière, Jean-Xavier Giroux, and Pierre Marquet "Label-free measurement of cell mechanical properties with digital holographic microscopy", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550T (5 March 2021); https://doi.org/10.1117/12.2577348
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KEYWORDS
Digital holography
Chemical mechanical planarization
Holography
Microscopy
Microfluidics
Control systems
Environmental sensing
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