Multicolor stimulated Raman scattering microscopy with a widely tunable portable light source

Maximilian Brinkmann; Thomas Würthwein; Tim Hellwig; Nick Lemberger; Carsten Fallnich

doi:10.1117/12.2578105

5 March 2021 Multicolor stimulated Raman scattering microscopy with a widely tunable portable light source

Maximilian Brinkmann, Thomas Würthwein, Tim Hellwig, Nick Lemberger, Carsten Fallnich

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560A (2021) https://doi.org/10.1117/12.2578105
Event: SPIE BiOS, 2021, Online Only

Abstract

We present high-speed multicolor stimulated Raman scattering imaging (SRS) enabled by an all-fiber light source. With a relative intensity noise level of -157 dBc above 10 MHz the light source is shot-noise limited up to a detector current of 0.75 mA. Compared to other fiber-based light sources optimized for SRS, the presented system is tunable in under 5 ms per arbitrary step between 700 and 3530 wavenumbers. The compact and environmentally stable system is predestined for fast multicolor assessments of medical or rapidly evolving samples with high chemical specificity.

Conference Presentation

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Maximilian Brinkmann, Thomas Würthwein, Tim Hellwig, Nick Lemberger, and Carsten Fallnich "Multicolor stimulated Raman scattering microscopy with a widely tunable portable light source", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560A (5 March 2021); https://doi.org/10.1117/12.2578105

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