Translator Disclaimer
Presentation
5 March 2021 Linking macroscopic rheology with molecular structure in fibrin hydrogels using coherent anti-Stokes Raman scattering (CARS) imaging
Author Affiliations +
Abstract
Fibrin is the polymerized protein responsible for the stabilizing mesh in blood coagulation. Its superior mechanical properties of being stiff or elastic if needed result from a hierarchical structure, including semiflexible single fibers as well as constituent protein restructuring. Here, we stretch and shear fibrin hydrogels to a regime where unfolding transitions of α-helical structures to β-sheet are induced and observe protein structural changes with spatially-resolved coherent Raman microscopy. We confirm the theoretically predicted orthogonal orientation of helices and sheets in strained networks. Spatially resolved structure protein maps reveal that the extent of structural transition changes with gel composition and becomes highly heterogeneous at large strain, indicative of substantial load-bearing inhomogeneity .
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mischa Bonn and Sapun Parekh "Linking macroscopic rheology with molecular structure in fibrin hydrogels using coherent anti-Stokes Raman scattering (CARS) imaging", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 1165610 (5 March 2021); https://doi.org/10.1117/12.2583182
PROCEEDINGS
PRESENTATION


SHARE
Advertisement
Advertisement
Back to Top