Engineered point spread functions enable extended depth for high throughput 3D imaging

Anurag Agrawal; Warren Colomb; Scott Gaumer; Anjul Loiacono

doi:10.1117/12.2579262

5 March 2021 Engineered point spread functions enable extended depth for high throughput 3D imaging

Anurag Agrawal, Warren Colomb, Scott Gaumer, Anjul Loiacono

Proceedings Volume 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII; 1165806 (2021) https://doi.org/10.1117/12.2579262
Event: SPIE BiOS, 2021, Online Only

Abstract

Confocal scanners are used to scan through a large sample depth necessary in both high throughput and high content screening applications. However, this scanning takes time and increases the cost and optical complexity of medical diagnostic devices. Here we present extended depth-of-field engineered point spread function (ePSF) technology, combined with our universal modular subsystem SPINDLE®, to enable precision extended-depth imaging that can be combined with high-content analysis (HCA), without the need for z scanning, and without trading off light or lateral resolution.

Conference Presentation

Citation Download Citation

Anurag Agrawal, Warren Colomb, Scott Gaumer, and Anjul Loiacono "Engineered point spread functions enable extended depth for high throughput 3D imaging", Proc. SPIE 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII, 1165806 (5 March 2021); https://doi.org/10.1117/12.2579262

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