Presentation + Paper
5 March 2021 Distinguishing small molecules with molecular laser polarization
Author Affiliations +
Abstract
Microlasers have emerged as a promising approach for the detection or identification of different biomolecules. Most lasers were designed to reflect changes of molecular concentration within the cavity, without being able to characterize biophysical changes in the gain medium. Here, we report a strategy to extract and amplify polarized laser emissions from small molecules and demonstrate how molecular rotation interplays with lasing at the nanoscale. The concept of molecular lasing polarization was proposed and was first evidenced to increase accordingly as the fluorophore binds to larger biomolecules in a microcavity. By detecting the molecular rotational correlation time through stimulated emission, small molecules could be distinguished while conventional fluorescence polarization cannot. Theoretical models were developed to elucidate the underlying mechanisms. Finally, different types of small molecules were analyzed by adopting a Fabry-Pérot optofluidic laser. The results suggest an entirely new tool to quantify small molecules and guidance for laser emissions to characterize biophysical properties down to the molecular level.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zhiyi Yuan, Xin Cheng, Yunke Zhou, and Yu-Cheng Chen "Distinguishing small molecules with molecular laser polarization", Proc. SPIE 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII, 116580F (5 March 2021); https://doi.org/10.1117/12.2579180
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Molecular lasers

Polarization

Molecules

Laser development

Laser resonators

Molecular biology

Optical microcavities

Back to Top