A New Type Of Fiber Optic Biosensor Based On The Intrinsic Fluorescence Of Immobilized Flavoproteins

Otto S. Wolfbeis; Wolfgang Trettnak

doi:10.1117/12.963198

13 February 1990 A New Type Of Fiber Optic Biosensor Based On The Intrinsic Fluorescence Of Immobilized Flavoproteins

Otto S. Wolfbeis, Wolfgang Trettnak

Proceedings Volume 1172, Chemical, Biochemical, and Environmental Fiber Sensors; (1990) https://doi.org/10.1117/12.963198
Event: OE/FIBERS '89, 1989, Boston, United States

Abstract

We describe a new biosensor for monitoring the concentration of enzyme glucose, lactate, and other substrates that are metabolized by an oxidation process. The method is based on the finding that enzymes having FAD as a prosthetic group change their fluorescence during interaction with a substrate. Typical enzymes that have been studied include glucose oxidase (GOD), lactate mono-oxygenase (LMO), and cholesterol oxidase (ChOD). Their fluorescence is monitored via fiber optic light guides at wavelengths above 500 nm, following fluorescence excitation at around 410 - 450 nm. The relative fluorescence intensities of the enzymes vary to a large extent, being highest for LMO, and rather low for ChOD. Typical detection limits are in the 0.5 mM range for lactate and 1.5 mM for glucose at ambient oxygen pressure. A characteristic feature of this sensor is the narrow dynamic range which usually does not exceed 3 mM. This can be explained in terms of enzyme kinetics and diffusional processes. Unlike optical biosensors based on measurement of the intrinsic fluorescence of NADH, this sensor type has the advantages of full reversibility (because reduced FAD-based enzymes accept oxygen as a second substrate) and analytical wavelengths that are compatible with plastic or glass fiber optics. It is fairly simple in construction because the enzyme acts as both the recognition and transduction element. The method also has been applied successfully in an flow injection analysis-like type of arrangement.

Citation Download Citation

Otto S. Wolfbeis and Wolfgang Trettnak "A New Type Of Fiber Optic Biosensor Based On The Intrinsic Fluorescence Of Immobilized Flavoproteins", Proc. SPIE 1172, Chemical, Biochemical, and Environmental Fiber Sensors, (13 February 1990); https://doi.org/10.1117/12.963198

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