Design of a microfluidic device for the phase-contrast tomography of flowing cells

Massimiliano M. Villone; Erica Santonastaso; Pasquale Memmolo; Gianluca Trotta; Francesco Merola; Pier Luca Maffettone; Pietro Ferraro

doi:10.1117/12.2592327

20 June 2021 Design of a microfluidic device for the phase-contrast tomography of flowing cells

Massimiliano M. Villone, Erica Santonastaso, Pasquale Memmolo, Gianluca Trotta, Francesco Merola, Pier Luca Maffettone, Pietro Ferraro

Author Affiliations +

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117860C (2021) https://doi.org/10.1117/12.2592327
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.

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Massimiliano M. Villone, Erica Santonastaso, Pasquale Memmolo, Gianluca Trotta, Francesco Merola, Pier Luca Maffettone, and Pietro Ferraro "Design of a microfluidic device for the phase-contrast tomography of flowing cells", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117860C (20 June 2021); https://doi.org/10.1117/12.2592327

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