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The effect of ultrashort laser radiation parameters on the process of biomaterial formation based on dispersed media of bovine serum albumin and carbon nanotubes was studied. It is noted that, in contrast to the use of continuous laser radiation of comparable power, the composite nanomaterial is formed when lower temperatures are reached, which provides additional advantages and a smaller change in the protein structure. This effect can also be associated with a pulse repetition rate of 80 MHz, in the interval, which is about 12.5 ns, partial cooling of the material can occur, while this effect is sufficient for the formation of a biomaterial. The hardness of this material is comparable to that of native tissue. A strong twofold change in the elasticity of such a material in comparison with drying in air indicates the formation of an inner framework of carbon nanotubes. The possibility of this effect is also confirmed by spectral studies, according to which, at the used wavelength of 810 nm, the radiation is absorbed mainly by carbon nanotubes and only as a result of heat transfer is transmitted to bovine serum albumin and is spent on water evaporation. In vitro studies of cell growth in the presence of biomaterial were carried out by quantitative (MTT test) and qualitative (microscopy) methods. It was found that the number of cells grown on the composites exceeds the number of cells in the control after 72 hours of incubation. The cells on the composites formed a monolayer, their morphology does not differ from the morphology of the cells in the control. In vitro studies indicate a positive effect of the biomaterial on cell adhesion and proliferation and, consequently, on the possibility of their use for tissue regeneration.
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