Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Yunfeng Nie; Aikio Sanna; Annukka Kokkonen; Uusitalo Sanna; Teemu Sipola; Simonetta Grilli; Heidi Ottevaere

doi:10.1117/12.2593354

20 June 2021 Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope

Yunfeng Nie, Aikio Sanna, Annukka Kokkonen, Uusitalo Sanna, Teemu Sipola, Simonetta Grilli, Heidi Ottevaere

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Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117861E (2021) https://doi.org/10.1117/12.2593354
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

The effective detection of very low abundant biomarkers can enable fast diagnosis of many severe and disabling diseases (e.g. Alzheimer’s Disease) at an early stage. To develop a cost-efficient, super-sensitive optical fluorescence detection microscope, we have proposed an optical modelling approach to predict the signal-noise-ratio that considers various noise sources introduced by the components of the detection system. After the optimal design is identified, a tolerance analysis regarding typical perturbations is performed for further mechanical design and assembly. Finally, experiments have demonstrated a limit of detection at a low abundant concentration reaching 0.05 pmol/ml.

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Yunfeng Nie, Aikio Sanna, Annukka Kokkonen, Uusitalo Sanna, Teemu Sipola, Simonetta Grilli, and Heidi Ottevaere "Optical design and development of a cost-efficient, compact, super-sensitive scanning confocal fluorescence microscope", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117861E (20 June 2021); https://doi.org/10.1117/12.2593354

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