Fluorescence enhancement based on plasmonic nanoparticle structures on ferroelectric platforms for bioimaging applications

A. Puerto; C. López-Fernández; J. L. Bella; I. Elvira; G. Minguez-Vega; A. García-Cabañes; M. Carrascosa

doi:10.1117/12.2592401

20 June 2021 Fluorescence enhancement based on plasmonic nanoparticle structures on ferroelectric platforms for bioimaging applications

A. Puerto, C. López-Fernández, J. L. Bella, I. Elvira, G. Minguez-Vega, A. García-Cabañes, M. Carrascosa

Author Affiliations +

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117861V (2021) https://doi.org/10.1117/12.2592401
Event: SPIE Optical Metrology, 2021, Online Only

Conference Poster

Abstract

Ferroelectric platforms based on photovoltaic LiNbO3:Fe are a powerful tool for trapping and patterning of micro- and nano-particles. By using this tool, metallic structures have been assembled from Ag and Au nanoparticles on the surface of the ferroelectric crystal. Their plasmonic response has been checked through the measurement of the reflectance extinction spectra. Then, the fluorescence enhancement of relevant biomaterials such as DNA and spermatozoa tagged with a variety of fluorophores such as DAPI, fluorescein, or rhodamine B has been investigated. Relevant plasmonic enhancement extended along the visible spectral range have been obtained, particularly with Ag nanoparticle structures. The results suggest the potential of the obtained metallic structures to improve bio-imaging procedures. Finally, we have found that the metal nanoparticle patterns are able to induce an organization of the sperm cells along the nanoparticle fringe structures.

Conference Presentation

Citation Download Citation

A. Puerto, C. López-Fernández, J. L. Bella, I. Elvira, G. Minguez-Vega, A. García-Cabañes, and M. Carrascosa "Fluorescence enhancement based on plasmonic nanoparticle structures on ferroelectric platforms for bioimaging applications", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117861V (20 June 2021); https://doi.org/10.1117/12.2592401

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