Enhanced quantitative phase imaging in Mach-Zehnder interferometer-based digital holographic microscopy by modulation of the object illumination with an electrically focus tunable lens

Björn Kemper; Álvaro Barroso; Kai Eder; Anne Marzi; Sabrina Ritz; Angelos Ntovas; Jürgen Schnekenbürger; Steffi Ketelhut

doi:10.1117/12.2592534

20 June 2021 Enhanced quantitative phase imaging in Mach-Zehnder interferometer-based digital holographic microscopy by modulation of the object illumination with an electrically focus tunable lens

Björn Kemper, Álvaro Barroso, Kai Eder, Anne Marzi, Sabrina Ritz, Angelos Ntovas, Jürgen Schnekenbürger, Steffi Ketelhut

Author Affiliations +

Proceedings Volume 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V; 117860I (2021) https://doi.org/10.1117/12.2592534
Event: SPIE Optical Metrology, 2021, Online Only

Abstract

In laser based digital holographic microscopy (DHM) quantitative phase imaging (QPI) is affected by scattering and interference fringes due to internal reflections in the experimental setup. We present a concept for the reduction of such coherence induced disturbances. In our approach, the sample illumination light is modulated by an electrically focus tunable lens while series of digital off-axis holograms are recorded from which subsequently averaged QPI images are calculated. The concept is compatible with Mach-Zehnder interferometer-based off-axis DHM and capable for usage with commercial research microscopes. The performance is illustrated by results from living cells.

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Björn Kemper, Álvaro Barroso, Kai Eder, Anne Marzi, Sabrina Ritz, Angelos Ntovas, Jürgen Schnekenbürger, and Steffi Ketelhut "Enhanced quantitative phase imaging in Mach-Zehnder interferometer-based digital holographic microscopy by modulation of the object illumination with an electrically focus tunable lens", Proc. SPIE 11786, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V, 117860I (20 June 2021); https://doi.org/10.1117/12.2592534

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