Fluorescence microscopy with optical sectioning based on varifocal metalens

Hsin Yu Kuo; Cheng Hung Chu; Sunil Vyas; Yu-Hsin Chia; Takuo Tanaka; Yu-Jung Lu; Yuan Luo; Yi-You Huang; Din Ping Tsai

doi:10.1117/12.2595113

1 August 2021 Fluorescence microscopy with optical sectioning based on varifocal metalens

Hsin Yu Kuo, Cheng Hung Chu, Sunil Vyas, Yu-Hsin Chia, Takuo Tanaka, Yu-Jung Lu, Yuan Luo, Yi-You Huang, Din Ping Tsai

Proceedings Volume 11812, Molecular and Nano Machines IV; 118120B (2021) https://doi.org/10.1117/12.2595113
Event: SPIE Organic Photonics + Electronics, 2021, San Diego, California, United States

Abstract

The optical sectioning images of volumetric biological sample was obtained by varifocal metalens with Moiré effect in fluorescence microscopy system in conjunction with telecentricity and HiLo image processing method. The varifocal metalens is capable of changing the focal depth ranging from 10 mm to 125 mm by tuning the relative angle between its paired metasurfaces. The standard resolution target and fluorescent mircrosphere are imaged and analyzed; its lateral resolution and optical sectioning capability are 2.46 μm and 7.5 μm, respectively. Our study offers a demonstration, a solid foundation, for developing more compact optical microscopy systems based on metasurface optics.

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Hsin Yu Kuo, Cheng Hung Chu, Sunil Vyas, Yu-Hsin Chia, Takuo Tanaka, Yu-Jung Lu, Yuan Luo, Yi-You Huang, and Din Ping Tsai "Fluorescence microscopy with optical sectioning based on varifocal metalens", Proc. SPIE 11812, Molecular and Nano Machines IV, 118120B (1 August 2021); https://doi.org/10.1117/12.2595113

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KEYWORDS

Luminescence

Microscopy

Optical microscopy

Biomedical optics

Monochromatic aberrations

Microscopes

Tissues

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