The ability to fabricate your own microfluidic devices provides flexibility to alter the exact design of the device as the project develops. Here we present a concise fabrication process of reusable microscope-compatible microfluidic devices for biophotonic applications. The method requires commonly available components, such as a 3D printer or 3D pen, heat plate, sonicator, PDMS, and acetone. For high NA objective’s measurements, a cover glass can be inserted and replaced from the main microfluidic chamber. For our studies of the controlled bacterial biofilm formation, a laser beam is also delivered from the side of the microfluidic device with a fiber-coupled laser or from the bottom through an objective.
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