Monitoring the metabolic activities of murine breast cancer cells in 2D and 3D cultures using nonlinear micro-spectroscopy of intrinsic NAD(P)H

Anh Q. T. Cong; Rafaela M. Lima Pimenta; Jon Holy; Ahmed A. Heikal

doi:10.1117/12.2597512

1 August 2021 Monitoring the metabolic activities of murine breast cancer cells in 2D and 3D cultures using nonlinear micro-spectroscopy of intrinsic NAD(P)H

Anh Q. T. Cong, Rafaela M. Lima Pimenta, Jon Holy, Ahmed A. Heikal

Author Affiliations +

Proceedings Volume 11825, Ultrafast Nonlinear Imaging and Spectroscopy IX; 118250B (2021) https://doi.org/10.1117/12.2597512
Event: SPIE Optical Engineering + Applications, 2021, San Diego, California, United States

Abstract

Cellular metabolism and cell behavior are believed to be significantly different in two-dimensional (2D) cultures from that in vivo. Here, we investigate the environmental effects of the metabolic state of murine breast cancer cells line (4T1) in 2D monolayer and three-dimensional (3D) collagen matrix cultures using integrated two-photon (2P) micro-spectroscopy (FLIM) of intrinsic NAD(P)H autofluorescence. In addition, we examined the metabolic responses to two novel compounds, MD1 and TPPBr, that target cellular metabolism by disrupting monocarboxylate transporters (MCTs) or oxidative phosphorylation, respectively, using 2P-FLIM of intracellular NAD(P)H in 2D and 3D cultures. Integrating nonlinear microscopy and spectroscopy of intrinsic NAD(P)H with refined 3D tumor-matrix in vitro models is a promising approach towards in-depth understanding of the roles of metabolism and metabolic plasticity in tumor growth and metastatic behavior.

Conference Presentation

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Anh Q. T. Cong, Rafaela M. Lima Pimenta, Jon Holy, and Ahmed A. Heikal "Monitoring the metabolic activities of murine breast cancer cells in 2D and 3D cultures using nonlinear micro-spectroscopy of intrinsic NAD(P)H", Proc. SPIE 11825, Ultrafast Nonlinear Imaging and Spectroscopy IX, 118250B (1 August 2021); https://doi.org/10.1117/12.2597512

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