Towards correlative imaging of neuronal tissue by phase-contrast x-ray tomography and SEM

Marina Eckermann; Torben Ruhwedel; Wiebke Möbius; Tim Salditt

doi:10.1117/12.2596133

9 September 2021 Towards correlative imaging of neuronal tissue by phase-contrast x-ray tomography and SEM

Marina Eckermann, Torben Ruhwedel, Wiebke Möbius, Tim Salditt

Proceedings Volume 11840, Developments in X-Ray Tomography XIII; 1184005 (2021) https://doi.org/10.1117/12.2596133
Event: SPIE Optical Engineering + Applications, 2021, San Diego, California, United States

Abstract

The mammalian brain shows a complex and hierarchical architecture, whose assessment at all functionally relevant scales requires the establishment of multiomics approaches. In this work, we propose a correlative workflow, which is based on large-scale overview PC-CT scans using the extended beams offered by laboratory μCT sources and parallel beam synchrotron radiation (SR), with subsequent zooms into specific regions-of-interest using cone-beam recordings with nanofocused laboratory sources or SR, and finally SEM in controlled and wellidentified sub-volumes obtained before. We demonstrate the workflow at the example of rOTO-stained murine corpus callosum tissue, a brain region rich in myelinated nerve fibers. Based on two different and complementary techniques, PC-CT and scanning electron microscopy (SEM), we approach the establishment of a correlative imaging workflow. As we show here, the workflow can be applied (i) in a correlative study, in order to add further quantitative value, for instance, or (ii) in a multiscale approach, to which PC-CT can contribute volume throughput, while SEM can contribute resolution. The findings from this work demonstrate the complementary strength of each modality in terms of resolution (FIB-SEM) and FOV or volume throughput (PC-CT).

Conference Presentation

Citation Download Citation

Marina Eckermann, Torben Ruhwedel, Wiebke Möbius, and Tim Salditt "Towards correlative imaging of neuronal tissue by phase-contrast x-ray tomography and SEM", Proc. SPIE 11840, Developments in X-Ray Tomography XIII, 1184005 (9 September 2021); https://doi.org/10.1117/12.2596133

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