Correlative light microscopy and x-ray microtomography of ground sections of mineralised tissues

David Mills; Alan Boyde

doi:10.1117/12.2596447

9 September 2021 Correlative light microscopy and x-ray microtomography of ground sections of mineralised tissues

David Mills, Alan Boyde

Proceedings Volume 11840, Developments in X-Ray Tomography XIII; 1184010 (2021) https://doi.org/10.1117/12.2596447
Event: SPIE Optical Engineering + Applications, 2021, San Diego, California, United States

Abstract

We show exact correlation between light microscopy of historical ground section microscope slides and XMT and OCT scans of the prepared slides. Samples were selected to represent a wide range of sizes and skeletal and dental tissue types, including human femoral bone, human permanent teeth, dog carnassial tooth, narwhal mandible, black rhinoceros molars, sperm whale cementum and dentine, African elephant ivory, and prairie marmot molars. XMT was conducted using the QMUL Mucat2 system, nominal voxel size 25um, 90kV, 24 hours. Analysis used TomView, ImageJ and Drishti software. In each case we were able to match XMT and LM. We can now report mineralisation densities for all the calcified tissues in the context of LM imagery.

Conference Presentation

Citation Download Citation

David Mills and Alan Boyde "Correlative light microscopy and x-ray microtomography of ground sections of mineralised tissues", Proc. SPIE 11840, Developments in X-Ray Tomography XIII, 1184010 (9 September 2021); https://doi.org/10.1117/12.2596447

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