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4 May 2021 Metabolism of stem cell grown on heterogeneous tissue-engineered scaffolds
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Abstract
One of the promising approaches to the treatment of injured tissue is the application of bioengineering techniques based on the introduction of cells into the damage area. An application of cells alone does not provide a complete replacement of the tissue defect. Therefore, scaffolds are used allowing organization of the cells into a structure, which is capable of the total reproduction of the damaged tissue integrity. Great number of factors, which influence the cell behavior and tissue formation at the injury site when using scaffolds are known. Here, we analyzed the effect of structural heterogeneity of scaffolds on cellular behavior and metabolism. All scaffolds were obtained by two-photon polymerization technic. It was found that colonization of heterogeneous scaffolds was insignificant less than homogeneous ones. However, there were not dead cells on heterogeneous matrix. We found that the level of free and bound NAD(P)H for the cells on the heterogeneous and homogeneous scaffolds was differ. This can indicate a different contribution of glycolysis and oxidative phosphorylation intensity in stem cells seeded on two types of scaffolds.
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Vadim V. Elagin, Svetlana A. Rodimova, Nikita V. Minaev, Anastasia I. Shpichka, Maria M. Karabut, Elena V. Zagaynova, Peter S. Timashev, and Daria S. Kuznetsova "Metabolism of stem cell grown on heterogeneous tissue-engineered scaffolds", Proc. SPIE 11845, Saratov Fall Meeting 2020: Optical and Nanotechnologies for Biology and Medicine, 1184506 (4 May 2021); https://doi.org/10.1117/12.2589052
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