A microfluidic cytometer with integrated on-chip optical systems for white blood cell analysis

Xinyue Su; Tao Peng; Qin Li; Zewen Wei Sr.; Xuantao Su Sr.

doi:10.1117/12.2602522

9 October 2021 A microfluidic cytometer with integrated on-chip optical systems for white blood cell analysis

Xinyue Su, Tao Peng, Qin Li, Zewen Wei Sr., Xuantao Su Sr.

Proceedings Volume 11900, Optics in Health Care and Biomedical Optics XI; 119000J (2021) https://doi.org/10.1117/12.2602522
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Abstract

White blood cells are a significant part of immune system, which can prevent human body from infection and invasion of foreign invaders. The count and recognition of white blood cells plays an important role in modern clinical practice. There is an urgent need to modify the conventional methods (such as cytometry), which are time-consuming and labor-intensive for white blood cell counting. This paper describes a microfluidic cytometer for white blood cells integrated a three-dimensional hydrodynamic focusing system and on-chip optical components, which can realize single-cell fluid flow and single-cell detection. Through the experiment, the device achieves the hydrodynamic focusing of cell flow and the detection of side scatter and fluorescence. For classifying and counting of white blood cells, we further perform an experiment using blood samples and get fairly good results.

Conference Presentation

Citation Download Citation

Xinyue Su, Tao Peng, Qin Li, Zewen Wei Sr., and Xuantao Su Sr. "A microfluidic cytometer with integrated on-chip optical systems for white blood cell analysis", Proc. SPIE 11900, Optics in Health Care and Biomedical Optics XI, 119000J (9 October 2021); https://doi.org/10.1117/12.2602522

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available