Stabilization three-dimensional refractive-index reconstruction system of single suspension cell

Fei Liang; Yongxiang Feng; Huichao Chai; Wenan Liao; Wenhui Wang

doi:10.1117/12.2601968

9 October 2021 Stabilization three-dimensional refractive-index reconstruction system of single suspension cell

Fei Liang, Yongxiang Feng, Huichao Chai, Wenan Liao, Wenhui Wang

Proceedings Volume 11904, Plasmonics VI; 1190417 (2021) https://doi.org/10.1117/12.2601968
Event: SPIE/COS Photonics Asia, 2021, Nantong, JS, China

Conference Poster

Abstract

Live cell microscopy technology, especially cell three-dimensional refractive-index reconstruction, is one of the important single-cell analysis methods, which can investigate subcellular organelles and biological mechanisms. Compared with the method of rotating the illumination light, rotating cells can obtain the complete internal structure information of the cell. However, cell rotation based reconstruction methods typically rotate cells in low operation throughput and image unstably in a low sensitivity. To bridge this gap, we demonstrate a combination strategy that uses acoustically oscillating bubble array based acoustofluidic device to simultaneously rotate cells in large-scale, and sets up a wDPM module to record cell quantitative phase maps with a high temporal and spatial phase sensitivity due to the common-path geometry and white light illumination. Result shows the phase accuracy of this system is less than 6.7%, the phase spatial consistency does not exceed 4%.

Conference Presentation

Citation Download Citation

Fei Liang, Yongxiang Feng, Huichao Chai, Wenan Liao, and Wenhui Wang "Stabilization three-dimensional refractive-index reconstruction system of single suspension cell", Proc. SPIE 11904, Plasmonics VI, 1190417 (9 October 2021); https://doi.org/10.1117/12.2601968

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