5-ALA-induced death dynamics and pathways of HeLa cells in vitro depending on FBS content and irradiation dose

Daria A. Gorbenko; Andrey V. Belashov; Anna A. Zhikhoreva; Iliya K. Litvinov; Anna V. Salova; Tatyana N. Belyaeva; Elena S. Kornilova; Irina V. Semenova; Oleg S. Vasyutinskii

doi:10.1117/12.2615650

3 December 2021 5-ALA-induced death dynamics and pathways of HeLa cells in vitro depending on FBS content and irradiation dose

Daria A. Gorbenko, Andrey V. Belashov, Anna A. Zhikhoreva, Iliya K. Litvinov, Anna V. Salova, Tatyana N. Belyaeva, Elena S. Kornilova, Irina V. Semenova, Oleg S. Vasyutinskii

Author Affiliations +

Proceedings Volume 11921, Emerging Technologies for Cell and Tissue Characterization; 119210R (2021) https://doi.org/10.1117/12.2615650
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

We report experiments on photodynamic treatment of HeLa cells with 5-ALA. Intracellular localization of generated PpIX was determined and combinations of treatment parameters providing major pathways of cell death were obtained using digital holographic tomography.

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Daria A. Gorbenko, Andrey V. Belashov, Anna A. Zhikhoreva, Iliya K. Litvinov, Anna V. Salova, Tatyana N. Belyaeva, Elena S. Kornilova, Irina V. Semenova, and Oleg S. Vasyutinskii "5-ALA-induced death dynamics and pathways of HeLa cells in vitro depending on FBS content and irradiation dose", Proc. SPIE 11921, Emerging Technologies for Cell and Tissue Characterization, 119210R (3 December 2021); https://doi.org/10.1117/12.2615650

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