Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy

J. Lightley; F. Görlitz; S. Kumar; R. Kalita; A. Kolbeinsson; E. Garcia; Y. Alexandrov; V. Bousgouni; R. Wysoczanski; P. Barnes; L.. Donelly; C. Bakal; C. Dunsby; M. A. A. Neil; S. Flaxman; P. M. W. French

doi:10.1117/12.2615663

7 December 2021 Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy

J. Lightley, F. Görlitz, S. Kumar, R. Kalita, A. Kolbeinsson, E. Garcia, Y. Alexandrov, V. Bousgouni, R. Wysoczanski, P. Barnes, L.. Donelly, C. Bakal, C. Dunsby, M. A. A. Neil, S. Flaxman, P. M. W. French

Author Affiliations +

Proceedings Volume 11922, Advances in Microscopic Imaging III; 1192205 (2021) https://doi.org/10.1117/12.2615663
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

We present a robust, low-cost neural network-based optical autofocus system that can operate over a range of ±100μm with submicron precision, enabling automated high-content super-resolved imaging with a 1.3 NA objective lens.

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J. Lightley, F. Görlitz, S. Kumar, R. Kalita, A. Kolbeinsson, E. Garcia, Y. Alexandrov, V. Bousgouni, R. Wysoczanski, P. Barnes, L.. Donelly, C. Bakal, C. Dunsby, M. A. A. Neil, S. Flaxman, and P. M. W. French "Robust optical autofocus system utilizing neural networks applied to automated multiwell plate STORM microscopy", Proc. SPIE 11922, Advances in Microscopic Imaging III, 1192205 (7 December 2021); https://doi.org/10.1117/12.2615663

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