Photobleaching and correlation spectroscopy for in-situ quantification of multi-labelled molecules on surfaces

Julius Sefkow-Werner; Irene Wang; Catherine Picart; Elisa Migliorini; Antoine Delon

doi:10.1117/12.2615697

7 December 2021 Photobleaching and correlation spectroscopy for in-situ quantification of multi-labelled molecules on surfaces

Julius Sefkow-Werner, Irene Wang, Catherine Picart, Elisa Migliorini, Antoine Delon

Author Affiliations +

Proceedings Volume 11922, Advances in Microscopic Imaging III; 119220F (2021) https://doi.org/10.1117/12.2615697
Event: European Conferences on Biomedical Optics, 2021, Online Only

Abstract

We quantify multi-labelled streptavidin on a surface with un-known distribution of fluorophores in situ using correlation spectroscopy and photobleaching. This will allow the characterization of the functionalization of biomaterials for future regenerative medicine applications.

Citation Download Citation

Julius Sefkow-Werner, Irene Wang, Catherine Picart, Elisa Migliorini, and Antoine Delon "Photobleaching and correlation spectroscopy for in-situ quantification of multi-labelled molecules on surfaces", Proc. SPIE 11922, Advances in Microscopic Imaging III, 119220F (7 December 2021); https://doi.org/10.1117/12.2615697

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