Lensless digital holographic microscope for label-free imaging

Manoj Kumar; Osamu Matoba; Mitsuhiro Morita; Yasuhiro Awatsuji

doi:10.1117/12.2615657

27 October 2021 Lensless digital holographic microscope for label-free imaging

Manoj Kumar, Osamu Matoba, Mitsuhiro Morita, Yasuhiro Awatsuji

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250G (2021) https://doi.org/10.1117/12.2615657
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

A new configuration of lensless digital holographic microscope based on a wedge plate is demonstrated. The system is simple in geometry, compact in size and highly stable in temporal phase measurements leading to the accurate investigations of minute membrane fluctuations of the biological cells. The proposed system is used for label-free quantitative phase imaging of the human red blood cells (RBCs) and the investigations of morphology and physiological parameters related to the phase information. The obtained results confirm the feasibility and effectiveness of the proposed system.

Citation Download Citation

Manoj Kumar, Osamu Matoba, Mitsuhiro Morita, and Yasuhiro Awatsuji "Lensless digital holographic microscope for label-free imaging", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250G (27 October 2021); https://doi.org/10.1117/12.2615657

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