Background signal rejection for pO2 measurements using adaptive optics and two-photon phosphorescence lifetime microscopy

Qi Pian; Baoqiang Li; Ikbal Sencan; Xiaojun Cheng; Jay Dubb; Xinyue Huang; Buyin Fu; Sergei A. Vinogradov; David A. Boas; Sava Sakadžic

doi:10.1117/12.2609110

7 March 2022 Background signal rejection for pO₂ measurements using adaptive optics and two-photon phosphorescence lifetime microscopy

Qi Pian, Baoqiang Li, Ikbal Sencan, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Sergei A. Vinogradov, David A. Boas, Sava Sakadžic

Author Affiliations +

Proceedings Volume 11946, Neural Imaging and Sensing 2022; 1194605 (2022) https://doi.org/10.1117/12.2609110
Event: SPIE BiOS, 2022, San Francisco, California, United States

Conference Poster

Abstract

We report the implementation and demonstration of an imaging system that combines two-photon phosphorescence lifetime microscopy (2PLM) and adaptive optics for improving oxygen partial pressure (pO₂) measurement accuracy in deep cortical layers of mice. The technique retrieves the background signal by forming an aberrated torus focus on the sample plane with an optical phase mask imposed on the system wavefront and subtracts it from normal phosphorescence emission signal. The proposed method is validated with intravascular pO₂ measurements from six mice imaged at up to ~600 μm depth.

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Qi Pian, Baoqiang Li, Ikbal Sencan, Xiaojun Cheng, Jay Dubb, Xinyue Huang, Buyin Fu, Sergei A. Vinogradov, David A. Boas, and Sava Sakadžic "Background signal rejection for pO₂ measurements using adaptive optics and two-photon phosphorescence lifetime microscopy", Proc. SPIE 11946, Neural Imaging and Sensing 2022, 1194605 (7 March 2022); https://doi.org/10.1117/12.2609110

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