In this work, we compare the performance of a quantitative scientific complementary metal-oxide semiconductor
(qCMOS) camera to the sCMOS camera for multiphoton imaging of tissue specimens. We find that the qCMOS
achieves a signal-to-background ratio that is ~2x and ~1.6x higher than that achieved by the sCMOS for twophoton
fluorescence and second-harmonic generation (SHG) imaging, respectively. The field-of-view of the
qCMOS camera is noticeably larger at ~1.3x that of the sCMOS. We also confirm that the qCMOS can spatially
resolve features as fine as 12.5 μm in 200-μm thick tendon tissue, at a penetration depth of 140 μm, using SHG
imaging. Our results highlight the applicability of the qCMOS for some multiphoton imaging applications.
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Mbaye Diouf, Zixi Lin, Krishangi Krishna, Kimani C. Toussaint Jr., "Multiphoton imaging using a quantitative CMOS camera," Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, 119650D (3 March 2022); https://doi.org/10.1117/12.2610510