Multiphoton imaging using a quantitative CMOS camera

Mbaye Diouf; Zixi Lin; Krishangi Krishna; Kimani C. Toussaint Jr.

doi:10.1117/12.2610510

3 March 2022 Multiphoton imaging using a quantitative CMOS camera

Mbaye Diouf, Zixi Lin, Krishangi Krishna, Kimani C. Toussaint Jr.

Proceedings Volume 11965, Multiphoton Microscopy in the Biomedical Sciences XXII; 119650D (2022) https://doi.org/10.1117/12.2610510
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

In this work, we compare the performance of a quantitative scientific complementary metal-oxide semiconductor (qCMOS) camera to the sCMOS camera for multiphoton imaging of tissue specimens. We find that the qCMOS achieves a signal-to-background ratio that is ~2x and ~1.6x higher than that achieved by the sCMOS for twophoton fluorescence and second-harmonic generation (SHG) imaging, respectively. The field-of-view of the qCMOS camera is noticeably larger at ~1.3x that of the sCMOS. We also confirm that the qCMOS can spatially resolve features as fine as 12.5 μm in 200-μm thick tendon tissue, at a penetration depth of 140 μm, using SHG imaging. Our results highlight the applicability of the qCMOS for some multiphoton imaging applications.

Conference Presentation

Citation Download Citation

Mbaye Diouf, Zixi Lin, Krishangi Krishna, and Kimani C. Toussaint Jr. "Multiphoton imaging using a quantitative CMOS camera", Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, 119650D (3 March 2022); https://doi.org/10.1117/12.2610510

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