Utilizing quantitative phase imaging with digital holographic microscopy for characterization of nanomaterial-induced tissue alterations

Angelos Ntovas; Álvaro Barroso; Kai Eder; Steffi Ketelhut; Antje Vennemann; Martin Wiemann; Jürgen Schnekenburger; Björn Kemper

doi:10.1117/12.2609625

2 March 2022 Utilizing quantitative phase imaging with digital holographic microscopy for characterization of nanomaterial-induced tissue alterations

Angelos Ntovas, Álvaro Barroso, Kai Eder, Steffi Ketelhut, Antje Vennemann, Martin Wiemann, Jürgen Schnekenburger, Björn Kemper

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Proceedings Volume 11970, Quantitative Phase Imaging VIII; 119700D (2022) https://doi.org/10.1117/12.2609625
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We explored the capabilities of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) to quantify nanoparticle-induced tissue alterations and the performance of DHM QPI for imaging hematoxylin-eosin-stained samples. In a pilot study, paraffin and cryosections from nanomaterial-laden rat lungs and vehicle-treated control tissue were analyzed. Our results from rat lungs show that the tissue average refractive index and morphology parameters extracted from QPI images allow the quantification of visible tissue changes due to toxic nanomaterials. Moreover, our results demonstrate that DHM with near-infrared laser light enables high quality QPI of samples with common histology staining.

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Angelos Ntovas, Álvaro Barroso, Kai Eder, Steffi Ketelhut, Antje Vennemann, Martin Wiemann, Jürgen Schnekenburger, and Björn Kemper "Utilizing quantitative phase imaging with digital holographic microscopy for characterization of nanomaterial-induced tissue alterations", Proc. SPIE 11970, Quantitative Phase Imaging VIII, 119700D (2 March 2022); https://doi.org/10.1117/12.2609625

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