Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry

Anne Marzi; Ilona Nordhorn; Kai Eder; Martin Wiemann; Uwe Karst; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2609700

2 March 2022 Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry

Anne Marzi, Ilona Nordhorn, Kai Eder, Martin Wiemann, Uwe Karst, Björn Kemper, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; 1197209 (2022) https://doi.org/10.1117/12.2609700
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Nanoparticles are currently the subject of numerous research activities due to their unique physical and chemical properties, such as for industrial applications, but also in studies on environmental toxicity and human health effects. Since the toxicity of nanoparticles is mainly based on their interaction and uptake by cells, the aim of this study was to quantify their cellular internalization. Quantitative phase imaging (QPI) has proven to be a versatile method for minimally invasive label-free imaging of biological specimens, whereas laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) allows the label-free identification and quantification of nanomaterials in biological tissues. However, the determination of nanoparticle concentrations within single cells in vitro is challenging for LA-ICP-MS as precise information about the cell volume is required. We thus combined the LA-ICP-MS analysis with digital holographic microscopy (DHM), a QPI technology, which allows single cell volume determination prior to measurements by LA-ICP-MS. The implementation of this method at the single cell level in vitro was investigated by determining the concentration of Ce in RLE-6TN lung epithelial cells after exposition to CeO2 nanomaterial. Results from correlative LA-ICP-MS and DHM QPI investigations on nanoparticle-loaded cells show significant effects on intracellular Ce-levels for RLE-6TN cells treated with different CeO2 nanoparticle concentrations and therefore demonstrate the feasibility of this new concept.

Conference Presentation

Citation Download Citation

Anne Marzi, Ilona Nordhorn, Kai Eder, Martin Wiemann, Uwe Karst, Björn Kemper, and Jürgen Schnekenburger "Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry", Proc. SPIE 11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, 1197209 (2 March 2022); https://doi.org/10.1117/12.2609700

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available