DNA damage and altered gene expression in cultured human skin fibroblasts exposed to 193-nm excimer laser radiation

Dvorit Samid; Denise M. Flessate; Alexandra C. Miller; Donata Rimoldi

doi:10.1117/12.17637

1 June 1990 DNA damage and altered gene expression in cultured human skin fibroblasts exposed to 193-nm excimer laser radiation

Dvorit Samid, Denise M. Flessate, Alexandra C. Miller, Donata Rimoldi

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Proceedings Volume 1202, Laser-Tissue Interaction; (1990) https://doi.org/10.1117/12.17637
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Tissue ablation using 193nm excimer lasers is being considered for a variety of surgical procedures, yet little is known regarding the potential mutagenic risk to human cells. The effects of sublethal doses of radiation on cellular DNA and gene expression have been examined in cultured human skin fibroblasts. Northern blot analysis of mRNA revealed an increase in the levels of the c-f. proto-oncogene, interstitial collagenase, and metallothionein transcripts after laser radiation at either 193nm or 248nm. Similar changes in gene expression have been previously observed in cells treated with different carcinogens, including classical UV light (254nm) and phorbol esters. In contrast to the conventional UV light or laser radiation at 248nm, the 193nm radiation did not cause significant pyrimidine dimer formation, as determined by measurements of unscheduled DNA synthesis. However, both 193nm and 248nm radiation induced micronuclei formation, indicative of chromosome breakage. These data indicate that exposure of actively replicating human skin cells to sublethal doses of 193nm laser radiation may result in molecular changes associated with carcinogenesis.

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Dvorit Samid, Denise M. Flessate, Alexandra C. Miller, and Donata Rimoldi "DNA damage and altered gene expression in cultured human skin fibroblasts exposed to 193-nm excimer laser radiation", Proc. SPIE 1202, Laser-Tissue Interaction, (1 June 1990); https://doi.org/10.1117/12.17637

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KEYWORDS

Skin

Excimer lasers

Ultraviolet radiation

Laser tissue interaction

Laser ablation

Laser irradiation

Radiation effects

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