Diagnosis of tumors by fluorescence: quantification of photosensitizer concentration

A. Edward Profio; Shusen Xie; Kuang-Hsien Shu

doi:10.1117/12.17646

1 July 1990 Diagnosis of tumors by fluorescence: quantification of photosensitizer concentration

A. Edward Profio, Shusen Xie, Kuang-Hsien Shu

Proceedings Volume 1203, Photodynamic Therapy: Mechanisms II; (1990) https://doi.org/10.1117/12.17646
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

The concentration of Photofrmn II is needed to determine the dose in photodynamic therapy, and in fluorescence diagnosis of tumors. A method based on fluorescence was devised which shows promise. Diffusion theory was applied in slab geometry with closely spaced excitation and emission wavelengths. A function of the measured radiant energy fluences for excitation and emission, and the depth x, was found that canceled the dependence on depth. A function of the diffusion coefficient and attenuation coefficient was derived from the measured diffuse reflectance. This function compensated for differences in absorption and scattering in different tissues, or a calibration phantom and tissue. Comparisons should be made with the concentration derived in other ways, but the fluorescence method shows promise.

Citation Download Citation

A. Edward Profio, Shusen Xie, and Kuang-Hsien Shu "Diagnosis of tumors by fluorescence: quantification of photosensitizer concentration", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17646

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