1 July 1990 Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation
Author Affiliations +
Proceedings Volume 1203, Photodynamic Therapy: Mechanisms II; (1990); doi: 10.1117/12.17679
Event: OE/LASE '90, 1990, Los Angeles, CA, United States
Previous biophysical investigations1 demonstrated photosensitive propertieB of EPIRUBICIN with sufficient photochemical stability significant radical production and sufficient maintanance of pharmacological properties like intercalation potency Previous in-vitro investigations2 (human bladder cancer line ATCC 5637) demonstrated after drug treatment an enhancement of cytotoxic activity with continoüs irradiation (100 mW/cm2, 20 mm) performed at the wavelength of maximal absorption of the drug Because of a high tissue absorption of this short wavelength combined with decreasing photochemical reactions in deeper tissue, we investigated whether the reaction rate can be increased using pulsed irradiation with an averaged light power of also 100 mW/cm2. As light sources we used an Ar2-laser and an Excimer dye laser system. To simulate different tissue depths we exposed monolayer cells in a first step with different averaged intensities (100, 10, 1 mW/cm2 ) and in a second step we additionally varied drug concentrations (50, 5 ug/ml). 24 h after EPIRUBICIN treatment using a concentration of 50 ug/ml, viability of pulsed irradiated cells was 2 fold less compared to irradiated cells.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Manfred Steinmetz, Heyke Cacile Diddens, "Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); doi: 10.1117/12.17679; https://doi.org/10.1117/12.17679

Tissue optics

Bladder cancer

Photodynamic therapy


In vitro testing




Comparison of two phthalocyanines for PDT
Proceedings of SPIE (January 31 1996)
In vitro study of the effects of Congo Red on...
Proceedings of SPIE (June 01 1991)
Photothermal effect in the tissue during photodynamic therapy
Proceedings of SPIE (February 02 2006)

Back to Top