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1 July 1990Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation
Previous biophysical investigations1 demonstrated photosensitive
propertieB of EPIRUBICIN with sufficient photochemical stability
significant radical production and sufficient maintanance of
pharmacological properties like intercalation potency
Previous in-vitro investigations2 (human bladder cancer line ATCC
5637) demonstrated after drug treatment an enhancement of cytotoxic activity with continoüs irradiation (100 mW/cm2, 20 mm)
performed at the wavelength of maximal absorption of the drug
Because of a high tissue absorption of this short wavelength combined with decreasing photochemical reactions in deeper tissue,
we investigated whether the reaction rate can be increased using
pulsed irradiation with an averaged light power of also
100 mW/cm2. As light sources we used an Ar2-laser and an Excimer
dye laser system.
To simulate different tissue depths we exposed monolayer cells in
a first step with different averaged intensities (100, 10, 1
mW/cm2 ) and in a second step we additionally varied drug concentrations (50, 5 ug/ml).
24 h after EPIRUBICIN treatment using a concentration of
50 ug/ml, viability of pulsed irradiated cells was 2 fold less
compared to irradiated cells.
Manfred Steinmetz andHeyke Cacile Diddens
"Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17679
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Manfred Steinmetz, Heyke Cacile Diddens, "Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation," Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17679