Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation

Manfred Steinmetz; Heyke Cacile Diddens

doi:10.1117/12.17679

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1 July 1990 Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation

Manfred Steinmetz, Heyke Cacile Diddens

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Proceedings Volume 1203, Photodynamic Therapy: Mechanisms II; (1990) https://doi.org/10.1117/12.17679
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Previous biophysical investigations1 demonstrated photosensitive propertieB of EPIRUBICIN with sufficient photochemical stability significant radical production and sufficient maintanance of pharmacological properties like intercalation potency Previous in-vitro investigations2 (human bladder cancer line ATCC 5637) demonstrated after drug treatment an enhancement of cytotoxic activity with continoüs irradiation (100 mW/cm2, 20 mm) performed at the wavelength of maximal absorption of the drug Because of a high tissue absorption of this short wavelength combined with decreasing photochemical reactions in deeper tissue, we investigated whether the reaction rate can be increased using pulsed irradiation with an averaged light power of also 100 mW/cm2. As light sources we used an Ar2-laser and an Excimer dye laser system. To simulate different tissue depths we exposed monolayer cells in a first step with different averaged intensities (100, 10, 1 mW/cm2 ) and in a second step we additionally varied drug concentrations (50, 5 ug/ml). 24 h after EPIRUBICIN treatment using a concentration of 50 ug/ml, viability of pulsed irradiated cells was 2 fold less compared to irradiated cells.

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Manfred Steinmetz and Heyke Cacile Diddens "Enhanced toxicity in chemotherapeutic drug Epirubicin-treated human bladder cancer cells after pulsed versus continuous irradiation", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17679

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