Dynamics of DNA/intercalator complexes

J. Michael Schurr; Pengguang Wu; Bryant S. Fujimoto

doi:10.1117/12.17702

1 May 1990 Dynamics of DNA/intercalator complexes

J. Michael Schurr, Pengguang Wu, Bryant S. Fujimoto

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17702
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Complexes of linear and supercoiled DNAs with different intercalating dyes are studied by time-resolved fluorescence polarization anisotropy using intercalated ethidium as the probe. Existing theory is generalized to take account of excitation transfer between intercalated ethidiums, and Forster theory is shown to be valid in this context. The effects of intercalated ethidium, 9-aminoacridine, and proflavine on the torsional rigidity of linear and supercoiled DNAs are studied up to rather high binding ratios. Evidence is presented that metastable secondary structure persists in dye-relaxed supercoiled DNAs, which contradicts the standard model of supercoiled DNAs.

Citation Download Citation

J. Michael Schurr, Pengguang Wu, and Bryant S. Fujimoto "Dynamics of DNA/intercalator complexes", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17702

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