1 May 1990 Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry
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Abstract
We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities of 0.6 - 200cP were obtained using propylene glycol at different temperatures (0-80°C) and methanol at 20°C. The intensity decays of naphthalene were measured in frequency-domain. Several theoretical models, including distance distributions were used to fit the data. The results indicate that end-to-end diffusion of flexible donor - acceptor pairs can be readily detected and quantified using frequency-domain fluorometry.
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Joseph R. Lakowicz, Wieslaw M. Wiczk, Ignacy Gryczynski, Henryk Szmacinski, Michael L. Johnson, "Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17748; https://doi.org/10.1117/12.17748
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