1 May 1990 Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry
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Abstract
We investigated the influence of end-to-end diffusion on intramolecular energy transfer between a naphthalene donor and dansyl acceptor linked by polymethylene chain. A range of viscosities of 0.6 - 200cP were obtained using propylene glycol at different temperatures (0-80°C) and methanol at 20°C. The intensity decays of naphthalene were measured in frequency-domain. Several theoretical models, including distance distributions were used to fit the data. The results indicate that end-to-end diffusion of flexible donor - acceptor pairs can be readily detected and quantified using frequency-domain fluorometry.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joseph R. Lakowicz, Joseph R. Lakowicz, Wieslaw M. Wiczk, Wieslaw M. Wiczk, Ignacy Gryczynski, Ignacy Gryczynski, Henryk Szmacinski, Henryk Szmacinski, Michael L. Johnson, Michael L. Johnson, } "Influence of end-to-end diffusion on intramolecular energy transfer as observed by frequency-domain fluorometry", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); doi: 10.1117/12.17748; https://doi.org/10.1117/12.17748
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