Comparable to annual rings present in a tree trunk, human tooth cementum contains yearly deposited incremental layers often termed incremental lines, which are generally visualized from tooth slides with optical microscopy in two dimensions. These micrometer-thin incremental lines are used to decode age-at-death and stress periods over the lifetime of an individuum. One can also visualize these layers without physical slicing by means of hard X rays because of density modulations. Within this project, two optically almost transparent tooth slides were used to record optical data in two dimensions with submicron pixel sizes. These data were registered with projections of available synchrotron radiation-based tomography data of the slides. Such data were also acquired for an entire tooth to determine thickness variations in each layer, the intra-layer thickness, and variations between the layers, the inter-layer thickness, automatically.
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