Confocal bioimaging the living cornea with autofluorescence and specific fluorescent probes

Barry R. Masters; Stephen W. Paddock

doi:10.1117/12.17793

1 August 1990 Confocal bioimaging the living cornea with autofluorescence and specific fluorescent probes

Barry R. Masters, Stephen W. Paddock

Proceedings Volume 1205, Bioimaging and Two-Dimensional Spectroscopy; (1990) https://doi.org/10.1117/12.17793
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Confocal bioimaging of the fine structure of the living rabbit cornea with both reflected light and fluorescent light has been demonstrated with a laser scanning confocal imaging system. Kalman averaging was used to reduce the noise in the images. Superficial epithelial, basal epithelial cells, stromal keratocytes, and endothelial cells were imaged. These cells and their subcellular structures were imaged in the two modes for comparison. The superficial epithelial cells were imaged by their autofluorescence (488/520 nm). This fluorescence signal may be due to the mitochondrial flavoproteins and can be used as a noninvasive indicator of cellular oxidative function. Thiazole orange was used to stain cell nuclei for fluorescence imaging. DiOC6 was used to stain the endoplasmic reticulum for fluorescence imaging. Fluorescein- conjugated phalloidin was used to stain actin for fluorescence imaging.

Citation Download Citation

Barry R. Masters and Stephen W. Paddock "Confocal bioimaging the living cornea with autofluorescence and specific fluorescent probes", Proc. SPIE 1205, Bioimaging and Two-Dimensional Spectroscopy, (1 August 1990); https://doi.org/10.1117/12.17793

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