1 July 1990 Confocal microscope with enhanced depth discrimination
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Confocal microscopy, in comparison to conventional microscopy, offers a narrower point-spread function, the rendition of phase structures in contrast, and high axial resolution. Of these, the capabilities of optical sectioning and the three-dimensional imaging of cells and tissues have attracted the most interest. The literature offers a number of studies exploring the factors affecting depth of field in confocal microscopy. In this article, the three-dimensional representation of fluorescence imagery is examined and the design of a laser scanning confocal fluorescence microscope with enhanced depth discrimination is described.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Roland V. Shack, Peter H. Bartels, Donald W. Hillman, M. Sabb, Richard L. Shoemaker, "Confocal microscope with enhanced depth discrimination", Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); doi: 10.1117/12.17804; https://doi.org/10.1117/12.17804


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