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1 July 1990 In-situ detection of viral nucleic acids using fluorescent probes
Richard M. Donovan
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Proceedings Volume 1206, New Technologies in Cytometry and Molecular Biology; (1990) https://doi.org/10.1117/12.17802
Event: OE/LASE '90, 1990, Los Angeles, CA, United States
Abstract
The objective of this work was to develop and improve technologies in cytometry and molecular biology for the specific in situ detection of viral nucleic acids. The major application for this system was the detection and measurement of individual cells stained specifically for the Human Immunodeficiency Virus (HIV) in patients with Acquired Immune Deficiency Syndrome (AIDS). Staining procedures used nucleic acid either directly or indirect labeled with enzymes or fluorescent probes. A cytometry system was used to acquire digitized images of labeled cells and determine their individual staining density or intensity. Efforts are underway to improve the sensitivity of these assays using time-resolved methods.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
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Richard M. Donovan "In-situ detection of viral nucleic acids using fluorescent probes", Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); https://doi.org/10.1117/12.17802
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KEYWORDS
Luminescence
Target detection
Molecular biology
Signal to noise ratio
Metals
Time resolved spectroscopy
Tissues
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