Efficiency of the phasor plot approach for the analysis of the antimicrobial properties of nanoparticles using two-photon microscopy

V. V. Nikolaev; D. A. Vrazhnov; A. S. Lozhkomoev

doi:10.1117/12.2616343

2 December 2021 Efficiency of the phasor plot approach for the analysis of the antimicrobial properties of nanoparticles using two-photon microscopy

V. V. Nikolaev, D. A. Vrazhnov, A. S. Lozhkomoev

Author Affiliations +

Proceedings Volume 12086, XV International Conference on Pulsed Lasers and Laser Applications; 120861J (2021) https://doi.org/10.1117/12.2616343
Event: XV International Conference on Pulsed Lasers and Laser Applications, 2021, Tomsk, Russian Federation

Abstract

Two-photon microscopy methods have been actively developing in the last two decades. In particular, various approaches are being developed to analyze metabolic activity obtained by laser microscopy with high temporal resolution. One such approach is ploting the autofluorescence lifetime signal data to the phasor plot and observe deviations from the normal state. This tool has proven itself well for analyzing the metabolic activity of biological objects, including bacteria. This study analyzes the antimicrobial activity for MRSA bacteria by phasor plot approach. It was possible to show that, the lifetime of autofluorescence in the phasor plane changes depending on the type of nanoparticles used, with which bacteria are incubated. This result can be used in the future for rapid assessment of the antimicrobial activity of nanoparticles.

Citation Download Citation

V. V. Nikolaev, D. A. Vrazhnov, and A. S. Lozhkomoev "Efficiency of the phasor plot approach for the analysis of the antimicrobial properties of nanoparticles using two-photon microscopy", Proc. SPIE 12086, XV International Conference on Pulsed Lasers and Laser Applications, 120861J (2 December 2021); https://doi.org/10.1117/12.2616343

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