1 April 1990 Picosecond fluorescence microscopy
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Abstract
A new picosecond time-resolved fluorescence microscope (PFM) has been developed for measuring and mapping the lifetime of laser excited fluorescence from a sample placed under a micro— scope. A compact laser diode is used to generate excitation pulses. The combination of a spectrograph and a streak camera performs time resolved fluorescence spectroscopy with a resolution of 40 ps ( 5 ps with deconvolusion). The lifetime and the intensity of laser excited fluorescence are analyzed b a computer and are displayed on a monitor as either a lifetime or intensity map.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Motoyuki Watanabe, Musubu Koishi, Hirofumi Kan, Kenneth J. Kaufmann, and Yutaka Tsuchiya "Picosecond fluorescence microscopy", Proc. SPIE 1209, Picosecond and Femtosecond Spectroscopy from Laboratory to Real World, (1 April 1990); doi: 10.1117/12.17901; https://doi.org/10.1117/12.17901
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