Robust estimation of 5-ALA-induced PpIX multiple-wavelength excitation fluorescence spectroscopy for improving glioma classification

A. Gautheron; M. Sdika; M. Hébert; B. Montcel

doi:10.1117/12.2620945

24 May 2022 Robust estimation of 5-ALA-induced PpIX multiple-wavelength excitation fluorescence spectroscopy for improving glioma classification

A. Gautheron, M. Sdika, M. Hébert, B. Montcel

Author Affiliations +

Proceedings Volume 12146, Clinical Biophotonics II; 1214607 (2022) https://doi.org/10.1117/12.2620945
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Protoporphyrin IX (PpIX) is a fluorophore now used to identify tumoral tissues. The tissue is usually excited at one wavelength, e.g., 405 nm, and the fluorescence signal generated by this molecule and other fluorophores (the baseline) is used to estimate the amount of PpIX. However, fluorophores too close to PpIX impair the estimation and resulting classifications. Thus, we handle this issue by suggesting an efficient multi-excitation wavelengths method, free from any a priori on the baseline. Our method aims to distinguish healthy tissues from tumor margins, while being more robust to baseline variability. It keeps an ability to distinguish healthy from tumor tissues up to 87% in cases where existing methods’ ability drops near 0%.

Citation Download Citation

A. Gautheron, M. Sdika, M. Hébert, and B. Montcel "Robust estimation of 5-ALA-induced PpIX multiple-wavelength excitation fluorescence spectroscopy for improving glioma classification", Proc. SPIE 12146, Clinical Biophotonics II, 1214607 (24 May 2022); https://doi.org/10.1117/12.2620945

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