1 July 1990 Apodization without loss of resolution in the 3-D reconstruction of icosohedral virions
Author Affiliations +
Abstract
ICOS is a modular software system for the 3-dimensional reconstruction of icosohedral particles from 2-dimensional projections. Noise in the resulting reconstructions is due to many sources including ringing artifact due to finite sampling of Fourier transforms and is comparable with many of the desired components representing particulate structure. This paper describes a method for reducing ringing artifact without significant loss of resolution. Application of a Blackman window to the original Fourier transforms significantly improves the signal to noise ratio by suppressing ringing artifact but reduces resolution in the fmal reconstruction. Taking a voxel by voxel minimum of the filtered and unfiltered 3-dimensional reconstruction restores the original resolution while retaining the enhanced SNR. 2.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Peter D. Lauren, Michael B. Merickel, William W. Newcomb, Jay C. Brown, "Apodization without loss of resolution in the 3-D reconstruction of icosohedral virions", Proc. SPIE 1233, Medical Imaging IV: Image Processing, (1 July 1990); doi: 10.1117/12.18918; https://doi.org/10.1117/12.18918
PROCEEDINGS
7 PAGES


SHARE
Back to Top